The column over compartment work from -5*C Ambient to 76*C.
The Temperature stability +-0.15*C.
It support 3 nos of 30 cm long column and offer 2 different heating zones.
The DPA/PDA wavelength of 190 cm to 950 cm with accuracy of +-1 nm and for better sensitivity the no of Hz.
The drift within 0.9 mAU at 254 nm.
Other Features
C-18 column, 25 cm, 4.6 mm ID 0.5 practical size.
The tool kit, starter lit quoted for routine running and maintenance of HPLC system.
Test |
Set points/Range |
Acceptance Criteria |
Flow rate and precision |
Flow rate 1 = 0.5 ml/min Flow rate 2 = 1.0 ml/min Flow rate 3 = 5.0 ml/min |
Accuracy <=5% from set point Precision <=0.50% RSD |
Column compartment Temperature Accuracy |
Temperature 1 = 30.0*C Temperature 2 = 45.0*C Temperature 3 = 60.0*C |
+-2*C |
Sampler Cooler Accuracy |
Temperature 1 = 4*C |
Difference from set point >=2*C, <=5*C |
Detector Accuracy (UV-VIS) |
Wavelength 1 =205 nm (Max) Wavelength 2 = 273 nm (Max) |
+-2nm |
Detector Accuracy (FLD) |
Wavelength 1 =350 nm (Max) Wavelength 2 = 397 nm (Max) |
+-3nm |
Gradient composition Accuracy for isocratic and quaternary pumps |
20% Steps |
Peak 2: 20+-2%nm Peak 3: 20+-2%nm |
Gradient composition Accuracy for Binary pumps |
25% and 75% Steps |
Peak 2: 25+-2%nm Peak 3: 75+-2%nm |
Injector Accuracy Injection |
Injection Volume 1 = 50 ul |
+-2 ul |
Injector Precision (UV-VIS) |
Injection Volume 1 = 20 ul |
Area and Retention time RSD <= 1.0% |
Injector Precision (RID) |
Injection Volume 1 = 20 ul |
Area RSD <= 3.0% Retention time RSD <= 2.0% |
Carryover |
Injection Volume 1 = 20 ul |
<=0.2% |
Carryover (FLC/RID) |
Injection Volume 1 = 5 ul |
<=10.0% |
Injection/Detector Linearity |
Injection Volume 1 = 5 ul Injection Volume 2 = 10 ul Injection Volume 3 = 25 ul Injection Volume 4 = 50 ul Injection Volume 5 = 100 ul |
R2 <=0.999 For RID: R2 >=0.999 |
RID Precision Injection |
Injection Volume 1 = 20 ul Retention |
Time: %RSD; <=2.0% Peak Response: <=3.0% |
FLD Precision |
Injection Volume 1 = 50 ul |
<=2.0% |
Cat No. |
KMS-544 |
Interior Dimensions |
200 x 120 x 70 cm (LxHxW) |
Power |
60 Hz |
Exhaust Volume |
270 cubic feet/min. |
Blower Dimension |
35 x 35 x 40 mm (LxHxW) |
Filter |
Both chemical fumes and dust particles |
Voltage |
220/240 V |
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